Charles S Wingo

Charles S Wingo, M.D.

PROFESSOR

Department: MD-NEPHROLOGY
Business Phone: (352) 273-8821
Business Email: cswingo@ufl.edu

Teaching Profile

Courses Taught
2017
BMS4905 Medical Sciences Senior Research

Board Certifications

  • Nephrology
    American Board of Internal Medicine

Clinical Profile

Specialties
  • Medicine
Subspecialties
  • Nephrology
Areas of Interest
  • Electrolytes
  • High blood pressure – adults

Publications

2022
Kidney-specific KO of the circadian clock protein PER1 alters renal Na+ handling, aldosterone levels, and kidney/adrenal gene expression.
American journal of physiology. Renal physiology. 322(4):F449-F459 [DOI] 10.1152/ajprenal.00385.2021. [PMID] 35129370.
2021
Benzamil-mediated urine alkalization is caused by the inhibition of H+-K+-ATPases.
American journal of physiology. Renal physiology. 320(4):F596-F607 [DOI] 10.1152/ajprenal.00444.2020. [PMID] 33554781.
2020
A noninvasive method to study the evolution of extracellular fluid volume in mice using time-domain nuclear magnetic resonance.
American journal of physiology. Renal physiology. 319(1):F115-F124 [DOI] 10.1152/ajprenal.00377.2019. [PMID] 32475134.
2020
Differences in renal BMAL1 contribution to Na+ homeostasis and blood pressure control in male and female mice.
American journal of physiology. Renal physiology. 318(6):F1463-F1477 [DOI] 10.1152/ajprenal.00014.2020. [PMID] 32338037.
2020
EDN1-AS, A Novel Long Non-coding RNA Regulating Endothelin-1 in Human Proximal Tubule Cells.
Frontiers in physiology. 11 [DOI] 10.3389/fphys.2020.00209. [PMID] 32231591.
2020
Knockout of the circadian clock protein PER1 results in sex-dependent alterations of ET-1 production in mice in response to a high-salt diet plus mineralocorticoid treatment.
Canadian journal of physiology and pharmacology. 98(9):579-586 [DOI] 10.1139/cjpp-2019-0688. [PMID] 32437627.
2020
NBCe1-A is required for the renal ammonia and K+ response to hypokalemia.
American journal of physiology. Renal physiology. 318(2):F402-F421 [DOI] 10.1152/ajprenal.00481.2019. [PMID] 31841393.
2019
Direct and indirect inhibition of the circadian clock protein Per1: effects on ENaC and blood pressure.
American journal of physiology. Renal physiology. 316(5):F807-F813 [DOI] 10.1152/ajprenal.00408.2018. [PMID] 30759025.
2019
Female C57BL/6J mice lacking the circadian clock protein PER1 are protected from nondipping hypertension.
American journal of physiology. Regulatory, integrative and comparative physiology. 316(1):R50-R58 [DOI] 10.1152/ajpregu.00381.2017. [PMID] 30427705.
2019
TRPV4 deletion protects against hypokalemia during systemic K+ deficiency.
American journal of physiology. Renal physiology. 316(5):F948-F956 [DOI] 10.1152/ajprenal.00043.2019. [PMID] 30838874.
2018
Renal Na-handling defect associated with PER1-dependent nondipping hypertension in male mice.
American journal of physiology. Renal physiology. 314(6):F1138-F1144 [DOI] 10.1152/ajprenal.00546.2017. [PMID] 29357420.
2017
Desoxycorticosterone pivalate-salt treatment leads to non-dipping hypertension in Per1 knockout mice.
Acta physiologica (Oxford, England). 220(1):72-82 [DOI] 10.1111/apha.12804. [PMID] 27636900.
2017
ENaC activity in the cortical collecting duct of HKα1 H+,K+-ATPase knockout mice is uncoupled from Na+ intake.
American journal of physiology. Renal physiology. 312(6):F1073-F1080 [DOI] 10.1152/ajprenal.00401.2016. [PMID] 28179253.
2017
Obstructive Nephropathy Without Hydronephrosis: Suspicion Is the Key.
Urology. 101:e9-e10 [DOI] 10.1016/j.urology.2016.11.041. [PMID] 28011273.
2016
Aldosterone alters the chromatin structure of the murine endothelin-1 gene.
Life sciences. 159:121-126 [DOI] 10.1016/j.lfs.2016.01.019. [PMID] 26775567.
2016
Alkaline activation of ClC-K2 chloride channels switches renal cells from reabsorbing to secreting.
The Journal of general physiology. 148(3):195-9 [DOI] 10.1085/jgp.201611669. [PMID] 27574289.
2015
An Integrated View of Potassium Homeostasis.
The New England journal of medicine. 373(1):60-72 [DOI] 10.1056/NEJMra1313341. [PMID] 26132942.
2015
An Integrated View of Potassium Homeostasis.
The New England journal of medicine. 373(18):1787-8 [DOI] 10.1056/NEJMc1509656. [PMID] 26510039.
2015
Effect of mineralocorticoid treatment in mice with collecting duct-specific knockout of endothelin-1.
American journal of physiology. Renal physiology. 309(12):F1026-34 [DOI] 10.1152/ajprenal.00220.2015. [PMID] 26400543.
2014
MicroRNA regulation of endothelin-1 mRNA in renal collecting duct cells.
Life sciences. 118(2):195-9 [DOI] 10.1016/j.lfs.2014.03.003. [PMID] 24632479.
2014
Tissue-specific and time-dependent regulation of the endothelin axis by the circadian clock protein Per1.
Life sciences. 118(2):255-62 [DOI] 10.1016/j.lfs.2014.03.028. [PMID] 24721511.
2013
A role for the circadian clock protein Per1 in the regulation of aldosterone levels and renal Na+ retention.
American journal of physiology. Renal physiology. 305(12):F1697-704 [DOI] 10.1152/ajprenal.00472.2013. [PMID] 24154698.
2013
An emerging role for microRNA in the regulation of endothelin-1.
Frontiers in physiology. 4 [DOI] 10.3389/fphys.2013.00022. [PMID] 23424003.
2013
Early progress in epigenetic regulation of endothelin pathway genes.
British journal of pharmacology. 168(2):327-34 [DOI] 10.1111/j.1476-5381.2012.01826.x. [PMID] 22220553.
2013
Endothelin-1 inhibits sodium reabsorption by ET(A) and ET(B) receptors in the mouse cortical collecting duct.
American journal of physiology. Renal physiology. 305(4):F568-73 [DOI] 10.1152/ajprenal.00613.2012. [PMID] 23698114.
2013
Hyperkalemia: getting to the heart of the matter.
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association – European Renal Association. 28(1):15-6 [DOI] 10.1093/ndt/gfs403. [PMID] 23028106.
2013
Introduction: potassium homeostasis in humans: our current understanding.
Seminars in nephrology. 33(3):205-6 [DOI] 10.1016/j.semnephrol.2013.04.001. [PMID] 23953796.
2013
Renal potassium homeostasis: a short historical perspective.
Seminars in nephrology. 33(3):209-14 [DOI] 10.1016/j.semnephrol.2013.05.001. [PMID] 23953798.
2013
The Effect of Aldosterone On the Mirna Content of a Murine Inner Medullary Collecting Duct Cell
The FASEB's Journal. 27
2012
A physiologic-based approach to the treatment of a patient with hypokalemia.
American journal of kidney diseases : the official journal of the National Kidney Foundation. 60(3):492-7 [DOI] 10.1053/j.ajkd.2012.01.031. [PMID] 22901631.
2012
Dexamethasone stimulates endothelin-1 gene expression in renal collecting duct cells.
Steroids. 77(5):360-6 [DOI] 10.1016/j.steroids.2011.12.011. [PMID] 22209709.
2012
Evaluation of Enac and Fluid Secretion Along the Mouse Cd: Endothelin-1 Down-Regulates Na Reabsorption
The FASEB's Journal. 26
2012
The circadian protein period 1 contributes to blood pressure control and coordinately regulates renal sodium transport genes.
Hypertension (Dallas, Tex. : 1979). 59(6):1151-6 [DOI] 10.1161/HYPERTENSIONAHA.112.190892. [PMID] 22526258.
2011
Endothelin-1 gene regulation.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 25(1):16-28 [DOI] 10.1096/fj.10-161612. [PMID] 20837776.
2011
Mineralocorticoids stimulate the activity and expression of renal H+,K+-ATPases.
Journal of the American Society of Nephrology : JASN. 22(1):49-58 [DOI] 10.1681/ASN.2010030311. [PMID] 21164026.
2011
Progesterone: not just a sex hormone anymore?
Kidney international. 80(3):231-3 [DOI] 10.1038/ki.2011.131. [PMID] 21760599.
2010
Heterogeneity of H-K-ATPase-mediated acid secretion along the mouse collecting duct.
American journal of physiology. Renal physiology. 298(2):F408-15 [DOI] 10.1152/ajprenal.00333.2009. [PMID] 19923411.
2010
Pharmacological profiles of the murine gastric and colonic H,K-ATPases.
Biochimica et biophysica acta. 1800(9):906-11 [DOI] 10.1016/j.bbagen.2010.05.002. [PMID] 20594946.
2010
Regulation of αENaC expression by the circadian clock protein Period 1 in mpkCCD(c14) cells.
Biochimica et biophysica acta. 1799(9):622-9 [DOI] 10.1016/j.bbagrm.2010.09.003. [PMID] 20868778.
2010
The renal H+-K+-ATPases: physiology, regulation, and structure.
American journal of physiology. Renal physiology. 298(1):F12-21 [DOI] 10.1152/ajprenal.90723.2008. [PMID] 19640897.
2010
The renal H,K-ATPases.
Current opinion in nephrology and hypertension. 19(5):478-82 [DOI] 10.1097/MNH.0b013e32833ce65f. [PMID] 20616716.
2009
Aldosterone modulates steroid receptor binding to the endothelin-1 gene (edn1).
The Journal of biological chemistry. 284(44):30087-96 [DOI] 10.1074/jbc.M109.030718. [PMID] 19638349.
2009
Narrative Review: Evolving Concepts in Potassium Homeostasis and Hypokalemia
. 150:619-U9
2009
Narrative review: evolving concepts in potassium homeostasis and hypokalemia.
Annals of internal medicine. 150(9):619-25 [PMID] 19414841.
2009
The circadian clock protein Period 1 regulates expression of the renal epithelial sodium channel in mice.
The Journal of clinical investigation. 119(8):2423-34 [DOI] 10.1172/JCI36908. [PMID] 19587447.
2008
Impaired acid secretion in cortical collecting duct intercalated cells from H-K-ATPase-deficient mice: role of HKalpha isoforms.
American journal of physiology. Renal physiology. 294(3):F621-7 [PMID] 18057185.
2007
Apical P2XR contribute to [Ca2+]i signaling and Isc in mouse renal MCD.
Biochemical and biophysical research communications. 359(3):438-44 [PMID] 17560948.
2007
Cellular distribution of the potassium channel KCNQ1 in normal mouse kidney.
American journal of physiology. Renal physiology. 292(1):F456-66 [PMID] 16896189.
2007
Downregulation of SGK1 by nucleotides in renal tubular epithelial cells.
American journal of physiology. Renal physiology. 293(5):F1751-7 [PMID] 17686958.
2007
The renal H+, K+-ATPases as therapeutic targets.
Expert opinion on therapeutic targets. 11(7):881-90 [PMID] 17614757.
2006
Characterization of the rabbit HKalpha2 gene promoter.
Biochimica et biophysica acta. 1759(10):443-50 [PMID] 17034876.
2004
A putative H+-K+-ATPase in the Atlantic stingray, Dasyatis sabina: primary sequence and expression in gills.
American journal of physiology. Regulatory, integrative and comparative physiology. 287(4):R981-91 [PMID] 15217793.
2004
Extracellular ATP-induced calcium signaling in mIMCD-3 cells requires both P2X and P2Y purinoceptors.
American journal of physiology. Renal physiology. 287(2):F204-14 [PMID] 15068972.
2004
Hydrogen peroxide mediates FK506-induced cytotoxicity in renal cells.
Kidney international. 65(1):139-47 [PMID] 14675044.
2003
Early transcriptional effects of aldosterone in a mouse inner medullary collecting duct cell line.
American journal of physiology. Renal physiology. 285(4):F664-73 [PMID] 12770840.
2003
Molecular modeling of the rabbit colonic (HKalpha2a) H+, K+ ATPase.
Journal of molecular modeling. 9(5):283-9 [PMID] 12884085.
2003
Quinine induced HUS-TTP: an unusual presentation.
The American journal of the medical sciences. 326(6):378-80 [PMID] 14671503.
2003
Renal microperfusion techniques.
Methods in molecular medicine. 86:457-73 [PMID] 12886788.
2002
Mechanisms through which ammonia regulates cortical collecting duct net proton secretion.
American journal of physiology. Renal physiology. 282(6):F1120-8 [PMID] 11997329.
2002
Molecular regulation of the HKalpha2 subunit of the H+,K(+)-ATPases.
Journal of nephrology. 15 Suppl 5:S54-60 [PMID] 12027222.
2002
Mutations in the putative pore-forming segment favor short-lived wild-type Kir2.1 pore conformations.
Biochemistry. 41(41):12457-66 [PMID] 12369836.
2002
Site-directed glycosylation tagging of functional Kir2.1 reveals that the putative pore-forming segment is extracellular.
The Journal of biological chemistry. 277(27):24382-9 [PMID] 11991952.
2001
Apical membrane of native OMCD(i) cells has nonselective cation channels.
American journal of physiology. Renal physiology. 281(1):F48-55 [PMID] 11399645.
2001
Immunohistochemical localization of H-K-ATPase alpha(2c)-subunit in rabbit kidney.
American journal of physiology. Renal physiology. 281(2):F357-65 [PMID] 11457728.
2001
Increased CO(2) stimulates K/Rb reabsorption mediated by H-K-ATPase in CCD of potassium-restricted rabbit.
American journal of physiology. Renal physiology. 281(2):F366-73 [PMID] 11457729.
2000
Activation of H(+)-K(+)-ATPase by CO(2) requires a basolateral Ba(2+)-sensitive pathway during K restriction.
American journal of physiology. Renal physiology. 279(1):F153-60 [PMID] 10894797.
2000
Effects of ammonia on bicarbonate transport in the cortical collecting duct.
American journal of physiology. Renal physiology. 278(2):F219-26 [PMID] 10662726.
2000
Proteinuria: potential causes and approach to evaluation.
The American journal of the medical sciences. 320(3):188-94 [PMID] 11014373.
1999
Apical proton secretion by the inner stripe of the outer medullary collecting duct.
The American journal of physiology. 276(4):F606-13 [DOI] 10.1152/ajprenal.1999.276.4.F606. [PMID] 10198421.
1999
Cation channels of the rabbit outer medullary collecting duct.
Seminars in nephrology. 19(5):472-6 [PMID] 10511386.
1999
H-K-ATPase in the RCCT-28A rabbit cortical collecting duct cell line.
The American journal of physiology. 276(2):F237-45 [DOI] 10.1152/ajprenal.1999.276.2.F237. [PMID] 9950954.
1999
Molecular identification of the renal H+,K+-ATPases.
Seminars in nephrology. 19(5):431-7 [PMID] 10511383.
1999
Treatment of hypokalemia.
The New England journal of medicine. 340(2):154-5; author reply 155 [PMID] 9917223.
1998
Chloride transport by the rabbit cortical collecting duct: dependence on H,K-ATPase.
Journal of the American Society of Nephrology : JASN. 9(12):2194-202 [PMID] 9848773.
1998
Hyperkalemia: a potential silent killer.
Journal of the American Society of Nephrology : JASN. 9(8):1535-43 [PMID] 9697678.
1998
Increased superoxide anion generation and altered vasoreactivity in rabbits on low-potassium diet.
The American journal of physiology. 274(6):H1955-61 [DOI] 10.1152/ajpheart.1998.274.6.H1955. [PMID] 9841522.
1997
Are academic medical societies needed in a changing healthcare arena?
The American journal of the medical sciences. 314(6):357-64 [PMID] 9413339.
1997
Hypokalemia–consequences, causes, and correction.
Journal of the American Society of Nephrology : JASN. 8(7):1179-88 [PMID] 9219169.
1997
The effects of potassium depletion and supplementation on blood pressure: a clinical review.
The American journal of the medical sciences. 314(1):37-40 [PMID] 9216439.
1995
Function and structure of H-K-ATPase in the kidney.
The American journal of physiology. 269(1 Pt 2):F1-16 [PMID] 7631822.
1995
In situ hybridization of H-K-ATPase beta-subunit mRNA in rat and rabbit kidney.
The American journal of physiology. 269(3 Pt 2):F345-54 [PMID] 7573483.
1995
Luminal acidification in K-replete OMCDi: inhibition of bicarbonate absorption by K removal and luminal Ba.
The American journal of physiology. 269(1 Pt 2):F116-24 [PMID] 7631825.
1995
Renal expression of the gene encoding the gastric H(+)-K(+)-ATPase beta-subunit.
The American journal of physiology. 268(3 Pt 2):F363-74 [PMID] 7900835.
1994
Luminal acidification in K-replete OMCDi: contributions of H-K-ATPase and bafilomycin-A1-sensitive H-ATPase.
The American journal of physiology. 267(3 Pt 2):F450-8 [PMID] 8092259.
1994
Stimulation of total CO2 flux by 10% CO2 in rabbit CCD: role of an apical Sch-28080- and Ba-sensitive mechanism.
The American journal of physiology. 267(1 Pt 2):F114-20 [PMID] 8048550.
1993
Case report: bacillary angiomatosis with massive visceral lymphadenopathy.
The American journal of the medical sciences. 306(4):236-40 [PMID] 8213892.
1993
Potassium transport in the kidney: regulation and physiological relevance of H+, K(+)-ATPase.
Seminars in nephrology. 13(2):213-24 [PMID] 8385362.
1993
Regulation of intracellular pH in two cell populations of inner stripe of rabbit outer medullary collecting duct.
The American journal of physiology. 265(3 Pt 2):F406-15 [PMID] 8214100.
1993
The renal H-K-ATPase: physiological significance and role in potassium homeostasis.
Annual review of physiology. 55:323-47 [PMID] 8385435.
1992
Evidence for the presence of a K-dependent acidifying adenosine triphosphatase in the rabbit renal medulla.
Kidney international. 42(5):1093-8 [PMID] 1333550.
1992
H-K-ATPase enhancement of Rb efflux by cortical collecting duct.
The American journal of physiology. 263(1 Pt 2):F43-8 [PMID] 1322055.
1992
Mechanisms of rubidium permeation by rabbit cortical collecting duct during potassium restriction.
The American journal of physiology. 263(6 Pt 2):F1134-41 [PMID] 1336311.
1992
Rubidium absorption and proton secretion by rabbit outer medullary collecting duct via H-K-ATPase.
The American journal of physiology. 263(5 Pt 2):F849-57 [PMID] 1332504.
1990
A prospective evaluation of a simplified captopril test for the detection of renovascular hypertension.
Archives of internal medicine. 150(3):569-72 [PMID] 2178583.
1990
Active and passive chloride transport by the rabbit cortical collecting duct.
The American journal of physiology. 258(5 Pt 2):F1388-93 [PMID] 2159724.
1990
H-K-ATPase immunoreactivity in cortical and outer medullary collecting duct.
Kidney international. 38(5):985-90 [PMID] 2176257.
1989
Active proton secretion and potassium absorption in the rabbit outer medullary collecting duct. Functional evidence for proton-potassium-activated adenosine triphosphatase.
The Journal of clinical investigation. 84(1):361-5 [PMID] 2544629.
1989
Potassium secretion by the cortical collecting tubule: effect of C1 gradients and ouabain.
The American journal of physiology. 256(2 Pt 2):F306-13 [PMID] 2916662.
1989
Reversible chloride-dependent potassium flux across the rabbit cortical collecting tubule.
The American journal of physiology. 256(4 Pt 2):F697-704 [PMID] 2705540.
1988
Diagnostic uses of angiotensin-converting enzyme inhibitors in renovascular hypertension.
American journal of hypertension. 1(4 Pt 2):344S-349S [PMID] 3056443.
1988
Loss of corticomedullary demarcation on magnetic resonance imaging: an index of biopsy-proven acute renal transplant dysfunction.
American journal of kidney diseases : the official journal of the National Kidney Foundation. 12(3):200-7 [PMID] 3046342.
1987
Picomole analysis of alkali metals by flameless atomic absorption spectrophotometry.
Kidney international. 31(5):1225-8 [PMID] 3599661.
1987
Potassium transport by medullary collecting tubule of rabbit: effects of variation in K intake.
The American journal of physiology. 253(6 Pt 2):F1136-41 [PMID] 3425723.
1986
Effect of acidosis on chloride transport in the cortical thick ascending limb of Henle perfused in vitro.
The Journal of clinical investigation. 78(5):1324-30 [PMID] 3771799.
1986
Effect of carbonic anhydrase inhibition on (U-B)pCO2 in the alkaline urine of the rabbit.
Renal physiology. 9(6):326-34 [PMID] 3110884.
1985
Cortical collecting tubule potassium secretion: effect of amiloride, ouabain, and luminal sodium concentration.
Kidney international. 27(6):886-91 [PMID] 2410660.
1985
Effects of in vitro aldosterone on the rabbit cortical collecting tubule.
Kidney international. 28(1):51-7 [PMID] 2413237.
1985
Glucocorticoid effects on Na-K-ATPase in rabbit nephron segments.
The American journal of physiology. 248(4 Pt 2):F487-91 [PMID] 2984945.
1984
Effect of ouabain on K secretion in cortical collecting tubules from adrenalectomized rabbits.
The American journal of physiology. 247(4 Pt 2):F588-95 [PMID] 6496687.
1982
Dietary modulation of active potassium secretion in the cortical collecting tubule of adrenalectomized rabbits.
The Journal of clinical investigation. 70(3):579-86 [PMID] 7107896.
1980
Simultaneous hepatic and renal involvement in acute syphilis. Case report and review of the literature.
Digestive diseases and sciences. 25(11):875-8 [PMID] 7002507.
1976
Letter: Ambulation after myocardial infarction.
The New England journal of medicine. 294(6):341-2 [PMID] 1246281.

Grants

Sep 2021 ACTIVE
Modulation of the renal aldosterone endothelin feedback system by the clock protein PER1
Role: Principal Investigator
Funding: NATL INST OF HLTH NIDDK
Sep 2017 ACTIVE
Per1 and the kidney clock in hypertension
Role: Co-Investigator
Funding: NATL INST OF HLTH NIDDK
Dec 2016 – Nov 2019
Novel mechanism to target the endothelin axis in aging and CKD
Role: Co-Investigator
Funding: NATL INST OF HLTH NIA
Dec 2016 – Sep 2017
IPA Irma Jeannette Matheny
Role: Other
Funding: US DEPT OF VET AFF GAINESVILLE MED CTR
Apr 2016 – Sep 2016
importing, transporting, quarantine, and continued breeding services with ATP12a mice
Role: Principal Investigator
Funding: US DEPT OF VET AFF CINCINNATI MED CTR
Oct 2014 – Sep 2016
IPA for Kit-Yan "Thomas" Cheng
Role: Principal Investigator
Funding: US DEPT OF VETERANS AFFAIRS
Oct 2013 – Sep 2016
IPA for Irma Jeanette Matheny
Role: Principal Investigator
Funding: US DEPT OF VET AFF GAINESVILLE MED CTR
Sep 2009 – Aug 2015
An Aldosterone-Endothelin Feedback Mechanism on Sodium Homeostasis.
Role: Principal Investigator
Funding: NATL INST OF HLTH NIDDK

Education

Fellowship – Nephrology
1981 · University of Texas
Residency – Internal Medicine
1979 · Dallas County Hospital
Residency – Internal Medicine
1978 · University of Texas
Medical Degree
1975 · Louisiana State University

Contact Details

Phones:
Business:
(352) 273-8821
Emails:
Business:
cswingo@ufl.edu
Addresses:
Business Mailing:
PO Box 100224
GAINESVILLE FL 32610
Business Street:
RM 580-1
1601 SW ARCHER RD RM 580-1
RESEARCH SERVICE (151)
GAINESVILLE FL 326081135